bt 549 tnbc cell lines  (ATCC)

Journal: Cellular & Molecular Biology Letters

Article Title: NSUN2–tRNA Val−CAC -axis-regulated codon-biased translation drives triple-negative breast cancer glycolysis and progression

doi: 10.1186/s11658-025-00781-z

Figure Lengend Snippet: NSUN2 promotes TNBC cell growth and metastasis in vitro. A – D The knockdown efficiency of NSUN2 in MDA-MB-231 and HCC1937 cells was assessed using qRT-PCR and western blotting. E – H The effects of NSUN2 knockdown on the proliferation ability of MDA-MB-231 and HCC1937 cells were evaluated using colony formation and CCK-8 assays. I , J The impacts of NSUN2 knockdown on the migration and invasion abilities of MDA-MB-231 and HCC1937 cells were assessed using Transwell assays. Scale bar = 200 μm, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human TNBC cell lines (HCC1937, MDA-MB-231, HCC1806, MDA-MB-468, and BT549) and a normal breast epithelial cell line (MCF10A) were all obtained from American Type Culture Collection (ATCC).

Techniques: In Vitro, Knockdown, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Migration

Journal: Cellular & Molecular Biology Letters

Article Title: NSUN2–tRNA Val−CAC -axis-regulated codon-biased translation drives triple-negative breast cancer glycolysis and progression

doi: 10.1186/s11658-025-00781-z

Figure Lengend Snippet: NSUN2 promotes TNBC cell growth and metastasis in vivo. A – C The effects of NSUN2 knockdown or overexpression on the in vivo growth of TNBC cells ( n = 6). D – E Representative images of HE staining of subcutaneous tumors in each group. F IHC analysis of NSUN2 and Ki67 expression in subcutaneous tumors from each group. G The metastatic sites notably increased in the NSUN2 overexpression group. Metastatic nodules in the lungs are labeled by arrows. H Representative images of HE staining of lung metastases. I IHC analysis of NSUN2 and Ki67 expression in lung metastases. Scale bar = 200 μm, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human TNBC cell lines (HCC1937, MDA-MB-231, HCC1806, MDA-MB-468, and BT549) and a normal breast epithelial cell line (MCF10A) were all obtained from American Type Culture Collection (ATCC).

Techniques: In Vivo, Knockdown, Over Expression, Staining, Expressing, Labeling

Journal: Cellular & Molecular Biology Letters

Article Title: NSUN2–tRNA Val−CAC -axis-regulated codon-biased translation drives triple-negative breast cancer glycolysis and progression

doi: 10.1186/s11658-025-00781-z

Figure Lengend Snippet: The NSUN2–tRNA Val−CAC axis modulates metabolic reprogramming to promote TNBC progression. A – E The impact of NSUN2 knockdown on glycolysis in MDA-MB-231 and HCC1937 cells. F – I Changes in glycolysis of HCC1806 cells after transfection with NSUN2-wt or NSUN2-mut plasmids. J – M qRT-PCR analysis of the expression levels of ALDH3A2, ALDH7A1, PFKM, and HK1 mRNA in MDA-MB-231 and HCC1937 cells after NSUN2 knockdown. N The mRNA expression levels of these four glycolysis-related genes in HCC1806 cells after transfection with NSUN2-wt or NSUN2-mut plasmids. O – R Polysome profiling qRT-PCR analysis of the TE of glycolysis-related genes in MDA-MB-231 cells following NSUN2 knockdown. S – V Polysome profiling qRT-PCR of the TE of glycolysis-related genes in HCC1806 cells after transfection with either NSUN2-wt or NSUN2-mut plasmids. W – X The protein expression levels of glycolysis-related genes were assessed. Y The correlation between NSUN2 expression and the expression levels of ADLH3A2, ALDH7A1, PFKM, and HK1 in TNBC tissues. Z IHC analysis of the effect of NSUN2 expression levels on the expression of glycolysis-related genes in subcutaneous tumors in nude mice. Scale bar = 200 μm, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human TNBC cell lines (HCC1937, MDA-MB-231, HCC1806, MDA-MB-468, and BT549) and a normal breast epithelial cell line (MCF10A) were all obtained from American Type Culture Collection (ATCC).

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Expressing

Journal: Cellular & Molecular Biology Letters

Article Title: NSUN2–tRNA Val−CAC -axis-regulated codon-biased translation drives triple-negative breast cancer glycolysis and progression

doi: 10.1186/s11658-025-00781-z

Figure Lengend Snippet: NSUN2 as a potential mediator of docetaxel resistance in TNBC. A The expression level of NSUN2 is positively correlated with the IC50 values of docetaxel in TNBC cell lines. B – C Dose–response curves of docetaxel in MDA-MB-231 and HCC1937 cells after knocking down NSUN2. D – E Colony formation assays were used to assess changes in the inhibitory effects of docetaxel on TNBC cells after NSUN2 knockdown. F The expression of NSUN2, ALDH3A2, ALDH7A1, PFKM, and HK1 in docetaxel-sensitive and docetaxel-resistant TNBC tissues was examined through IHC staining. G A schematic representation of the xenograft animal model. H – J Tumor weight and volume in nude mice after NSUN2 knockdown and/or docetaxel treatment. K IHC analysis of changes in NSUN2 and Ki67 expression levels after the respective treatments. Scale bar = 200 μm, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Human TNBC cell lines (HCC1937, MDA-MB-231, HCC1806, MDA-MB-468, and BT549) and a normal breast epithelial cell line (MCF10A) were all obtained from American Type Culture Collection (ATCC).

Techniques: Expressing, Knockdown, Immunohistochemistry, Animal Model

Journal: Cell Communication and Signaling : CCS

Article Title: Artificial intelligence-driven discovery of YH395A: A novel TGFβR1 inhibitor with potent anti-tumor activity against triple-negative breast cancer

doi: 10.1186/s12964-025-02337-2

Figure Lengend Snippet: YH395A inhibited breast cancer cell proliferation. (A) Breast cancer cell lines (MDA-MB-231, MDA-MB-468, BT549, SUM159, and BT-20), normal mammary epithelial cells (MCF-10 A), human gastric mucosal epithelial cells (GES-1), and human embryonic lung fibroblast cells (MRC-5) were treated with the indicated concentrations of YH395A or YR-290. After 96 h, SRB assay was performed. The bars indicate the mean ± SD. (B) Colony formation assays of breast cancer cells

Article Snippet: The MCF-10 A, GES-1, MRC-5, and triple-negative breast cancer (TNBC) cell lines BT549 (RRID: CVCL_0062), MDA-MB-468 (RRID: CVCL_0419), BT-20 (RRID: CVCL_0169), and MDA-MB-231(RRID: CVCL_0063) were sourced from the American Type Culture Collection (ATCC, USA), while SUM159 cells were generously donated by Dr. Yuzhu Zhang (Guangdong Academy of Chinese Medicine, China).

Techniques: Sulforhodamine B Assay